Our pens are dual-chamber, so the powder ships dry and sealed. Here's the activation sequence from the guide printed inside every lid.
Read first. The steps below follow the activation guide printed inside the lid of every box. They cover assembly, mixing and storage for research use only — they are not dosing instructions and not guidance for human or veterinary use.
The pen ships with dry powder in one chamber and liquid in the other. You must combine and dissolve them first, by twisting the pen to activate it and then rolling it gently until the solution is clear.
If you dial or press before the powder is fully dissolved, the internal piston pushes against a solid powder cake — the liquid path is still blocked, and the pen jams mechanically. Once that happens it can't be forced. So: twist to A, roll at B until clear, then attach the needle and prime at C.
Open the box and remove the pen components. Insert the dual-chamber cartridge into the pen body. Every box also contains 10 × 30G sterile needles and alcohol pads.
The pen activates through three twist positions, marked A, B and C. The first twist, to A, locks the dual-chamber cartridge into position and brings the liquid and powder chambers together. Do this before anything else.
Twist to B, then gently roll the pen between your palms for about 60 seconds, until the solution is completely clear with no visible powder or cloudiness. Do not shake. Do not dial or press until it is fully dissolved — dialing against undissolved powder is the most common cause of a jammed pen.
Once mixed, the compound must be stored in a refrigerator between 2°C and 8°C. Do not freeze. Reconstitute only when you're ready to use it — that's the point of the dual chamber.
Screw on a fresh needle from the box until it's fully seated — snug, not loose, and only the 30G needles supplied. Twist to C and prime: dial out until a drop appears at the needle tip. A small amount of overflow at this stage is normal — that's the priming volume clearing the air from the needle. A needle that's on too loosely, or an incompatible needle whose tip is too short, won't pierce the cartridge seal, so nothing flows and the pen feels stuck.
Still at position C, dial to your research units per your protocol. Clean the site with an alcohol pad, and dispose of needles and empty cartridges according to your local research-waste requirements.
Once the pen is activated, the peptide is dissolved into the pen's diluent to make a single solution. The dial turns in clicks. The full peptide volume is dispensed over 100 clicks; the pen keeps turning to 110, with the extra 10 clicks reserved for priming the needle, after which it's empty and no longer usable. The calculator works off the 100 peptide clicks. How much each click delivers depends on the pen's strength and its mixed volume (most pens hold 1.5 mL; the Retatrutide pen holds 1 mL), so pick your pen (or enter its figures) and everything below recalculates.
After mixing, the whole amount of peptide sits in the pen's total volume. Dividing one by the other gives the concentration — how much peptide is in every millilitre.
20 mg ÷ 1 mL = 20.00 mg/mLThe full pen dispenses over 100 clicks, so one click is the pen's volume divided by 100. Multiply that by the concentration to get the amount of peptide in one click.
1 mL ÷ 100 clicks = 0.010 mL per clickTo turn any amount in milligrams into clicks, divide by the amount per click. For example, on a 20 mg / 1.5 mL pen each click is 0.2 mg, so 2 mg works out to 10 clicks. The calculator below does it for whatever pen you've set above.
Based on 0.2 mg (0.010 mL) per click. Rounds to the nearest 0.1.
Verify your specific pen first. These figures assume the full peptide volume dispenses over 100 clicks (the pen dials to 110 in total, the last 10 reserved for priming). If your pen is calibrated differently, enter its real figures above before relying on any conversion. This is measurement information for research handling only — not dosing guidance, and not for human or veterinary use. Message us with the pen model if you'd like us to confirm the click calibration.
Almost every stuck pen comes down to one of the following. Work through them in order before assuming the pen is faulty.
This is the most common cause. If you dial or press while there's still solid powder in the cartridge, the piston pushes against a powder cake with nowhere for the liquid to go, and the pen locks up.
Fix: make sure the pen was activated (chambers combined) and rolled until the solution is completely clear before you dial. If it's already jammed from pressing early, stop — forcing it won't clear it. Message us.
If the needle isn't screwed all the way on, its inner point may not reach through and pierce the rubber seal at the front of the cartridge. The liquid path never opens, so pressing does nothing.
Fix: remove the needle and re-attach a fresh one, screwing it on until it's fully seated. Then prime again until a drop appears.
A needle whose base thread doesn't match, or whose tip is too short, won't reach the seal either — same result as a loose needle.
Fix: use only the 30G needles supplied in the box. If you've run out, message us before substituting another needle.
If the powder is fully dissolved, the needle is correctly seated and compatible, and it still won't press, the front path is blocked and the plunger is pushing against trapped liquid (a hydraulic lock). This can't be forced by hand — it's like trying to push a syringe with the cap still on.
Fix: don't force it. Take a short video showing the needle attached and the pen not depressing, and send it to us on WhatsApp so we can identify the cause and sort a replacement if needed.
Still stuck? Message us on WhatsApp at +66 95 914 6063. A short video of the pen with the needle attached helps us diagnose it fastest, and we'll sort a replacement if the pen is faulty.